METHODS AND COMPOSITIONS FOR DETECTING MICROBIAL PRODUCTION OF WATER-IMMISCIBLE COMPOUNDS
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United States of America Patent
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Issued Date -
Feb 27, 2014
app pub date -
May 10, 2012
filing date -
May 13, 2011
priority date (Note) -
Abandoned
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Abstract
Provided herein are methods and compositions useful for detecting the production of compounds in a cell, for example, a microbial cell genetically modified to produce one or more such compounds at greater yield and/or with increased persistence compared to a parent microbial cell that is not genetically modified. In some embodiments, the methods comprise contacting a solution with a fluorescent dye that directly binds the recombinantly produced compound, wherein the solution comprises a plurality of cells recombinantly producing the compound; and detecting the fluorescent dye under spectral conditions suitable for the selective detection of the fluorescent dye bound to the recombinantly produced compound.
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