Importance

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Abstract

A method for the detection and quantitation of analytes of interest and variants of the analyte of interest comprising the steps of:

(i) providing a sample containing an analyte of interest;(ii) spiking sample with known amount of calibrant;(iii) performing an LCMS or LCMSMS analysis on the spiked sample to produce a data set;(iv) determining from said data set the relative quantity of analyte of interest to calibrant;(v) calculating the absolute quantity of the analyte of interest from said relative quantity of analyte of interest and said known amount of calibrant;(vi) searching for one or more previously identified candidate sequences for one or more known variants denoted by one or more specific peaks to identify the presence of said one or more known variants within the sample;(vii) determining the relative quantity or amount of said one or more variants to said analyte of interest; and(viii) calculating the absolute quantity or amount of any of said one or more variants present in the sample.

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First Claim