Infectious Clones of RNA Viruses and Vaccines and Diagnostic Assays Derived Thereof
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United States of America Patent
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app pub date -
Sep 30, 2013
filing date -
Oct 30, 1996
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Abandoned
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Abstract
An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with the recombinant nucleic acid and selecting for infectious clones. The recombinant nucleic acid comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either. The infectious clones can be used in single or dual purpose vaccines and in viral vector vaccines.
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