Selection Method and Recombinant Microorganisms and uses Therefor
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United States of America Patent
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Issued Date -
Nov 28, 2013
app pub date -
May 22, 2013
filing date -
May 23, 2012
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Abandoned
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Abstract
One or more genes in a biosynthesis pathway for a vitamin or other essential nutrient which is needed for the survival of a microorganism can be used as an effective selective marker to identify cells transformed with an exogenous nucleic acid. The microorganism does not naturally contain or express the one or more gene. This permits genetic manipulations to be performed. It permits lower cost fermentations to be performed. It permits production of the essential nutrient for subsequent commodity use.
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