CELL CULTURING FORMULATION AND CULTURING AND QUANTIFICATION METHOD OF CD140B+ CELLS THEREOF
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United States of America Patent
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Issued Date -
Nov 21, 2013
app pub date -
Sep 13, 2012
filing date -
May 16, 2012
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Abstract
The present invention discloses a cell culturing formulation and a culturing and quantification method of CD140b+ cells thereof. The cell culturing formulation is applicable for inducing the growth of the CD140b+ cells in peripheral blood. The cell culturing formulation comprises a culturing medium, a serum, a mixed additive and a defined factor. Wherein, concentrations of the culturing medium, the serum, the mixed additive and the defined factor are 59˜98% (v/v), 0.1˜20% (v/v), 1˜10% (v/v) and 10−7˜10% (v/v) respectively.
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