MICROBIAL PRODUCTION OF POLYHYDROXYALKANOATES

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United States of America Patent

APP PUB NO 20130288323A1
SERIAL NO

13991383

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Abstract

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A process for producing medium-chain-length 3-hydroxyalkanoic acids in a liquid medium, the process comprising: (i) intermixing a liquid culture medium and a microbial inoculum comprising a pure culture of a Pseudomonas putida strain having a portion of a phaC1 gene deleted and/or a portion of a phaC2 gene deleted and/or a portion of a phaZ gene deleted, (ii) culturing the mixture for a period of time to allow for microbial cell growth to occur in the liquid culture medium thereby producing a spent liquid medium and a plurality of microbial cells wherein medium-chain-length 3-hydroxyalkanoic acids are synthesized, (iii) separating the medium-chain-length 3-hydroxyalkanoic acids from the plurality of microbial cells and the spent liquid medium; and (iv) recovering the separated medium-chain-length 3-hydroxyalkanoic acids. A pure culture comprising transgenic microbial cells wherein a portion of a phaC1 gene and/or a phaC2 gene and/or a phaZ gene has been deleted.

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Patent Owner(s)

Patent OwnerAddress
UNIVERSITY OF MANITOBAROOM 202 ADMINISTRATION BUILDING WINNIPEG R3T 2N2

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Levin, David B Winnipeg, CA 2 5
Sharma, Parveen Winnipeg, CA 1 1

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