USES OF SPATIAL CONFIGURATION TO MODULATE PROTEIN FUNCTION
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United States of America Patent
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Issued Date -
Oct 24, 2013
app pub date -
Jun 21, 2013
filing date -
Feb 28, 2001
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Abandoned
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Abstract
This invention provides a set of methods for modulating protein spatial configuration. First, select the amino-acid codon for encoding the target protein according to host codon usage. Second, choose combinations which can modulate the spatial configuration and construct into different vectors which can transfect a series of hosts. Third, choose the vector promoter by monitoring a combination of base pairs after combining the code sequence of the promoter and the target protein. Finally, choose the appropriate expression host to express the target protein, refold and purify, measure the activity and spatial configuration.
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