SUB-2 MICRON CHIRAL STATIONARY PHASE SEPARATION AGENTS FOR USE WITH SUPERCRITICAL FLUID CHROMATOGRAPHY
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Feb 27, 2013
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Apr 20, 2012
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Abstract
The present invention relates to chiral separation column for use with supercritical fluid chromatography (SFC) containing a porous sub-2 chiral stationary phase agent that offered significant savings in run times and solvent use over the more conventional chiral columns using SFC methods. It was surprisingly discovered that SFC columns containing highly stable and backpressure resistant sub-2 micron stationary phase agents which were either coated or at least partially covalently bonded with polysaccharide or derivatized polysaccharide and which have an average particle diameter less than 2 microns can be obtained by maintaining a pore ratio of from 0.0042 to about 0.010 provide improved efficiency.
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