METHOD TO DETERMINE AND CORRECT BASELINE AND TO CHARACTERIZE PCR AMPLIFICATION KINETICS
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United States of America Patent
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Oct 17, 2013
app pub date -
Mar 28, 2013
filing date -
Apr 16, 2012
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Abstract
The disclosed methods correct for high-sloped baseline observed in real-time PCR curves and provides a way of verifying amplification efficiency using a statistical approach. The method teaches the conversion of fluorescent data points to transform the baseline from sloped to horizontal. Then, a 4-parameter logistic model is applied to simulate the PCR kinetics. A cycle number corresponding to a template concentration can then be determined at a inflection point defined by the model
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