PRODUCTION OF POLIOVIRUS AT HIGH TITERS FOR VACCINE PRODUCTION
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United States of America Patent
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Issued Date -
Oct 17, 2013
app pub date -
Mar 15, 2013
filing date -
Jul 16, 2009
priority date (Note) -
Abandoned
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Abstract
Described is a process for producing poliovirus, the process comprising: a) providing a serum-free suspension culture of cells, which are primary human retina cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.
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