Compositions and Methods Relating to Variant DNA Polymerases and Synthetic DNA Polymerases
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United States of America Patent
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Issued Date -
Oct 3, 2013
app pub date -
Aug 31, 2012
filing date -
Sep 1, 2011
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Abandoned
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Abstract
Compositions of novel polymerase variants and methods of identifying, making and using these novel polymerases are described. The variants have been shown to have advantageous properties such as increased thermostability, deoxyuridine nucleoside triphosphate tolerance, salt tolerance, reaction speed and/or increased reverse transcriptase properties. Uses for these improved enzymes have been demonstrated in isothermal amplification such as LAMP. Enhanced performance resulting from the use of these variants in amplification has been demonstrated both in reaction vessels and in dedicated automated amplification platforms.
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