Functionalization Processes and Reactants Used in Such Processes Using an Isatoic Anhydride or a Derivative Thereof, Biological Molecules Thus Treated and Kits
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United States of America Patent
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Dec 5, 2011
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Dec 6, 2010
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Abstract
The present invention relates to a process of functionalising at least one ribonucleic acid (RNA) molecule which comprises the following steps:
- a) having at least: a binding molecule constituted by an isatoic anhydride or a derivative thereof,a group of interest, anda binding arm linking the binding molecule with the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group in: position 2′ of the ribose of one of the RNA nucleotides, and/orposition(s) 2′ and/or 3′ of the ribose of the nucleotide at the 3′ terminal end of the RNA, and c) obtaining an anthranilate linking, via the binding arm, the RNA to the group of interest.
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