System And Method For Generation And Use Of Compact Clonally Amplified Products

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United States of America Patent

APP PUB NO 20130217023A1
SERIAL NO

13765223

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Abstract

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A method for sequencing a nucleic acid is described that comprises the steps of: coupling an adaptor to at least one end of a template nucleic acid molecule; circularizing the adaptor coupled nucleic acid molecule; amplifying the adaptor coupled nucleic acid molecule to form a linear amplified concatamer molecule comprising a plurality of copies of the template nucleic acid molecule; compacting the linear amplified concatamer molecule with a branched polyelectrolyte species to form a branched polyelectrolyte compacted amplified concatamer molecule; and sequencing the branched polyelectrolyte compacted amplified concatamer molecule to produce a sequence composition of the template nucleic acid molecule.

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Patent Owner(s)

Patent OwnerAddress
454 LIFE SCIENCES CORPORATION20 COMMERCIAL STREET BRANFORD CT 06405

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Godwin, Brian Christopher North Haven, US 18 176
Mealmaker, Craig Elder New Haven, US 4 23
Shanbhag, Priya Rocky Hill, US 3 16

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