Assay for detecting free light chains by capillary zone electrophoresis
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United States of America Patent
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Apr 14, 2020
Grant Date -
Aug 15, 2013
app pub date -
Jun 2, 2011
filing date -
Jun 21, 2010
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Abstract
The invention provides a method detecting free light chains (FLCs) comprising: (i) providing a sample from a subject; (ii) mixing the sample with an anti-FLC specific antibody, or fragments thereof capable of specifically binding the FLC, to form a mixture; (iii) passing the mixture through a capillary tube by capillary zone electrophoresis (CZE); and (iv) detecting the presence of the antibody or fragment thereof after passage through at least a portion of the capillary tube. Capillary tubes for use in CZE and kits comprising capilliary tubes and at least one anti-FLC antibody are also provided.
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