METHOD FOR ACQUISITION OF SMALL RNA

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United States of America Patent

APP PUB NO 20130197206A1
SERIAL NO

13825587

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The purpose of the present invention is to provide a method which is applicable to high throughput and by which small RNA can be obtained simply and efficiently. The present invention relates to a method for acquisition of small RNA, comprising contacting a carrier, in which a substance having affinity to small RNA-binding protein is immobilized on its surface, with a complex of the small RNA-binding protein and a small RNA (protein-RNA complex) to bind the protein-RNA complex to the aforementioned carrier, and releasing the small RNA by heating the carrier bound with the aforementioned protein-RNA complex at 70° C. to 100° C. in water or buffer solution of pH 3.0 to pH 8.0

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Patent Owner(s)

Patent OwnerAddress
WAKO PURE CHEMICAL INDUSTRIES LTD1-2 DOSHOMACHI 3-CHOME CHUO-KU OSAKA-SHI OSAKA 540-8605

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hirayasu, Kazunari Amagasaki-shi, JP 10 66
Nishibu, Takahiro Amagasaki-shi, JP 7 19

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