METHOD FOR DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA USING DUPLEX POLYMERASE CHAIN REACTION
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United States of America Patent
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Issued Date -
Jul 11, 2013
app pub date -
May 26, 2011
filing date -
May 27, 2010
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Abandoned
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Abstract
Disclosed are primer sets specific for the IS6110 or 16S rRNA gene characteristic of MTC and for the 16S rRNA gene characteristic of NTM, kits for the detection of MTC and NTM, comprising the same, and methods for detecting MTC and NTM by duplex PCR using the same. Because the primers are exclusive to Mycobacterium tuberculosis and nontuberculous mycobacteria, the methods, which can employ an inexpensive typical PCR cycler, can be a clinical diagnostic means useful for the detection of both Mycobacterium tuberculosis and nontuberculous mycobacteria at the same time, with higher efficiency.
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