BIDIRECTIONAL PROMOTER REPORTER VECTOR FOR THE ANALYSIS OF DUAL REGULATORY ELEMENTS
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United States of America Patent
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Issued Date -
Jun 20, 2013
app pub date -
Dec 13, 2012
filing date -
Dec 15, 2011
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Abandoned
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Abstract
A bidirectional expression vector is described that can be utilized to determine the existence and characteristics of bidirectional promoters. The bidirectional expression vector includes two different reporter genes in a head to head (5′ to 5′) arrangement. In addition, the bidirectional expression vector can include a polylinker region located between the heads of the two reporter genes that provides multiple cloning sites for nonexclusive examination of polynucleotide sequences. The vector can also include a splicing site and drug resistance. The bidirectional expression vector can be used to examine a polynucleotide sequence for the presence of divergent regulator regions and, following determination of a bidirectional promoter, can be utilized to further elucidate characteristics of the bidirectional promoter.
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