IN SITU-DILUTION METHOD AND SYSTEM FOR MEASURING MOLECULAR AND CHEMICAL INTERACTIONS
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United States of America Patent
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Issued Date -
Jun 20, 2013
app pub date -
Jan 13, 2011
filing date -
Jan 13, 2010
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Abandoned
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Abstract
The present invention relates to a method for testing multiple analyte concentrations within a biosensor system through a single injection of sample. The method involves flowing a fluid sample containing a neat analyte concentration along a flow path in a fluid system and diluting the sample by causing it to merge with a fluid that is free of analyte in a second flow path under laminar flow conditions. The merged fluid stream is directed through a turbulent third flow path of a very low dead volume. The third flow path carries the merged fluid stream to a sensing region where the analyte is exposed to an immobilized ligand. The concentration of analyte can be controlled in this method by adjusting the flow rates of the sample flow and analyte-free fluid flow. A fluidic system for carrying out this method is also disclosed.
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