I-CREI VARIANTS WITH NEW SPECIFICITY AND METHODS OF THEIR GENERATION
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United States of America Patent
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Issued Date -
Jun 13, 2013
app pub date -
Apr 30, 2010
filing date -
Apr 30, 2010
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Abandoned
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Abstract
The present invention relates to 1-Cre1 variants which can in particular recognise and cleave DNA targets which do not comprise the same nucleotides at positions ±6 and ±7 which are present in the wild type 1-Cre1 target. The present invention also relates to 1-Cre1 variants which can recognise and cleave targets which do not comprise the wild type nucleotides at positions ±4, ±5, ±6, ±7 and to 1-Cre1 variants with new specificity which can recognise and cleave targets which do not comprise the wild type nucleotides at positions ±4, ±5, ±6, ±7, ±8, ±9 and ±10.
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