Compositions and Methods for Adenylating Oligonucleotides
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United States of America Patent
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Issued Date -
Jun 6, 2013
app pub date -
Apr 1, 2011
filing date -
Apr 1, 2010
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Abandoned
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Abstract
A method is provided for generating a preparation in which more than 70% of the oligonucleotides are adenylated. The method includes reacting an oligonucleotide with an ATP-sensitive ligase where the ligase is characterized by its ability to efficiently generate adenylated oligonucleotides at ATP concentrations at which ligation and circularization of the oligonucleotide is minimal.
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