Method of Producing a Lipid Bilayer and Microstructure and Measuring Arrangement
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United States of America Patent
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Issued Date -
Jun 6, 2013
app pub date -
Jun 1, 2011
filing date -
Jun 7, 2010
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a method of producing a lipid bilayer over a microcavity open on one side and to a microstructure for investigating lipid bilayers and an associated measuring arrangement. The method of producing a lipid bilayer over a microcavity open on one side comprises the following steps: filling the microcavity with an electrolyte solution; moving a fluid containing dissolved lipids in a first direction onto the microcavity; moving the fluid in a second direction away from the microcavity; monitoring the formation of the lipid bilayer over the microcavity by detecting an impedance between a counter-electrode connected to the fluid and a measuring electrode, which is arranged inside the microcavity. The microstructure has a substrate, in which at least one microcavity is formed, wherein at least one measuring electrode is arranged inside the microcavity and wherein the at least one microcavity can be connected to a fluid channel so that a laminar flow of fluid can be made to flow over the microcavity with at least two different directions of flow.
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