Method for Reducing Star Activity in Restriction Endonucleases
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United States of America Patent
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Issued Date -
May 23, 2013
app pub date -
Jan 10, 2012
filing date -
Aug 31, 2005
priority date (Note) -
Abandoned
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Abstract
Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
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