METHOD FOR SYNTHESIZING POLYHYDROXYALKANOATE USING A MICROORGANISM
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United States of America Patent
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Issued Date -
May 16, 2013
app pub date -
Nov 15, 2011
filing date -
Nov 15, 2011
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Abandoned
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Abstract
A method for synthesizing polyhydroxyalkanoate using a microorganism includes providing a Ralstonia eutropha strain; providing a fermentation tank and adding crude glycerol into the fermentation tank; adding a nitrogen source into the fermentation tank to adjust a carbon to nitrogen (C/N) ratio to 20:1 to 150:1; controlling temperature of the fermentation tank to a temperature ranging from 20 to 45° C.; adding 5 to 20% by volume of the Ralstonia eutropha strain into the fermentation tank using a mixing speed of from 100 to 500 rpm, an aeration quantity of 0.5-2 vvm and a concentration of dissolved oxygen of 30 to 80%; fermenting contents of the fermentation tank for 48 to 96 hours to allow the Ralstonia eutropha strain to proliferate and to allow polyhydroxyalkanoate to be synthesized from the crude glycerol within cells of the Ralstonia eutropha strain; and disrupting the cells of the Ralstonia eutropha strain and extracting polyhydroxyalkanoate.
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