Ubiquitination assay
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United States of America Patent
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Jul 17, 2018
Grant Date -
May 9, 2013
app pub date -
Apr 28, 2011
filing date -
Apr 29, 2010
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Abstract
The present invention related to a method of assaying ubiquitination in a sample by combining ubiquitin and two or more of E1, E2, E3 and a substrate protein in a sample under conditions suitable for ubiquitination to take place, exposing the sample with a labelled binding partner which is specific for the ubiquitin and measuring the amount of labelled ubiquitin bound to any one of the components in the sample, wherein one or more of the components in the sample comprises an immobilization tag which facilitates its immobilization onto a solid surface.
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