Perhydrolase variant providing improved specific activity
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United States of America Patent
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Jul 16, 2013
Grant Date -
Apr 25, 2013
app pub date -
Oct 22, 2012
filing date -
Oct 25, 2011
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Abstract
An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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