NUCLEIC ACID EXTRACTION METHOD, NUCLEIC ACID EXTRACTION REAGENT KIT, AND NUCLEIC ACID EXTRACTION REAGENT
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United States of America Patent
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Issued Date -
Mar 14, 2013
app pub date -
Aug 23, 2012
filing date -
Aug 26, 2011
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Abandoned
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Abstract
A method is provided that is suitable for various test samples and that prepares nucleic acid templates directly usable for gene amplification reaction such as PCR method or RT-PCR method conveniently and promptly, preferably under a mild condition. A nucleic acid extraction method is provided comprising a process of contacting a nucleic acid extraction reagent with a test sample, wherein the nucleic acid extraction reagent comprises zwitterionic buffer solution. Preferably, the nucleic acid extraction reagent comprises a surfactant and a proteolytic enzyme.
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