ALGAL TRANSFORMATION SYSTEMS, COMPOSITIONS AND METHODS
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United States of America Patent
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Issued Date -
Mar 14, 2013
app pub date -
Aug 24, 2012
filing date -
Aug 25, 2011
priority date (Note) -
Abandoned
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Abstract
One aspect of the invention relates to a method for introducing a cargo molecule into an algal cell, the method comprising preparing a composition comprising the cargo molecule and a cell penetrating peptide (CPP) and exposing the algal cell to the composition. The method may also comprise treating the algal cell to disrupt a cell wall of the algal cell. One aspect of the invention relates to a method for transforming an algal cell, the method comprising preparing a composition comprising a nucleic acid molecule and a cell penetrating peptide and exposing the algal cell to the composition. In some embodiments, the CPP is Transportan, Penetratin, an HIV Tat fragment, or a polyarginine, or a variant thereof.
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