BIOSENSORS COMPRISING PROTEIN-BINDING DOMAINS AND FLUORESCENT PROTEINS
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United States of America Patent
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Issued Date -
Mar 7, 2013
app pub date -
Apr 13, 2011
filing date -
Apr 13, 2010
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Abstract
The present invention provides compositions and methods for monitoring cellular signal transduction events. In particular, signal transduction events may be monitored by the use of biosensors comprising protein-binding domains, which bind signal-transduction proteins, and fluorescent proteins. Provided herein, therefore, methods for detecting activation of signal transduction proteins or screening for agents that modulate the activity of signal transduction proteins. Also provided are cells comprising the biosensors; lentiviral particles comprising biosensor coding sequence; and kits comprising the lentiviral particles.
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