Method for Amplification of Target Nucleic Acids Using a Multi-Primer Approach
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United States of America Patent
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Issued Date -
Feb 21, 2013
app pub date -
Aug 15, 2012
filing date -
Aug 17, 2011
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Abstract
The present invention is a method for amplification of target nucleic acids wherein a first and second primer set is used to amplify a target nucleic acid in a single amplification reaction. The primers of the first primer set generate a first amplification product that serves as a template for amplification by the primers of the second primer set due to the incorporation of a first and second tag sequence added to the target nucleic acid from the forward and reverse primers of the first primer set to which the primers of the second primer set can hybridize to its complement. Additional sequences are thereby added to the resulting target nucleic acid amplicons because of further amplification from the first amplification products by the primers of the second primer set.
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