METHOD FOR DETECTING AND IDENTIFYING CANDIDA SPECIES
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United States of America Patent
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Issued Date -
Feb 7, 2013
app pub date -
Jul 31, 2009
filing date -
Aug 18, 2008
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Abandoned
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Abstract
The present invention describes a method, based on a semi-nested polymerase chain reaction (PCR), for the detection and identification of ten clinically relevant Candida species. The method involves a first PCR amplification of the internal transcribed spacer region ITS2 of fungal ribosomal RNA, followed by a second PCR reaction with a species-specific primer. Custom-designed primers are provided.
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