METHOD FOR REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
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United States of America Patent
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Issued Date -
Jan 24, 2013
app pub date -
Jul 21, 2011
filing date -
Jul 21, 2011
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Abandoned
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Abstract
A method for reverse transcription polymerase chain reaction comprises steps: preparing a capillary, and adding a reverse transcription enzyme into the capillary; and performing a lyophilization process on the RT enzyme contained by the capillary to fabricate the RT enzyme into a lyophilized RT reagent in the capillary. Therefore, a buffer solution, an RNA sample, a polymerase and a primer solution can be added into the capillary to re-dissolve the lyophilized RT reagent and enable a reverse transcription reaction and a polymerase chain reaction of the RNA sample to directly take place inside the capillary, so as to promote convenience and efficiency of experiment.
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