METHODS, CASSETTES, GELS AND APPARATUSES FOR ISOLATION AND COLLECTION OF BIOMOLECULES FROM ELECTROPHORESIS GELS
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United States of America Patent
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app pub date -
Jul 16, 2012
filing date -
Aug 31, 2006
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Abstract
Electrophoresis systems, assemblies, cassettes and methods for easily, and more effectively and efficiently, isolating a biomolecule band from an electrophoretic gel are provided. The methods use an electrophoresis cassette with at least one loading well and at least one collection well. A sample containing the biomolecule of interest is placed into at least one loading well and buffer or water is placed in at least one collection well. An electric field is then applied to drive migration and separation of the sample into different component bands within the gel. When the component of interest is located within at least one collection well, the electric field is terminated and the buffer or water in the collection well is removed, thereby isolating and collecting the sample component of interest.
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