Primer And Probe For Detection Of Mycobacterium Intracellulare, And Method For Detection Of Mycobacterium Intracellulare Using The Same
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United States of America Patent
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May 23, 2012
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May 2, 2006
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Abstract
The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence shown in SEQ ID NOS: 1-8, or a part or the entire sequence of a sequence complementary to the nucleotide sequence shown in SEQ ID NOS: 1-8, wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence of Mycobacterium intracellulare gene; a primer or a probe for the detection of M. intracellulare, which comprises the oligonucleotide; and a method for detection of M. intracellulare using the primer and/or the probe. According to the detection method, false-positive results can be eliminated and detection of M. intracellulare can be carried out with higher accuracy, greater precision, and greater specificity compared to a conventional diagnostic method employing a cell culture assay or a PCR assay. The method also enables to quantify a microbial cell.
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