Method and reagents for identifying pluripotent stem cells
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United States of America Patent
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Issued Date -
Nov 29, 2012
app pub date -
May 21, 2012
filing date -
May 20, 2011
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Abstract
The present invention relates to methods for distinguishing pluripotent stem cells from partially differentiated, or spontaneously differentiated cells, and to reagents for use in such methods. In particular, the method enables the detection of alternatively spliced transcripts and the polypeptides encoded thereby, which are uniquely associated with, or present at a higher level in pluripotent stem cells than in cells which have partially differentiated. Reagents for use in the method include nucleic acids which bind the alternatively spliced transcript or which amplify the alternatively spliced transcript, and antibodies which bind the polypeptide product of the alternatively spliced transcript.
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