APTAMERS DIRECTED AGAINST THE MATRIX PROTEIN-1 OF TYPE A INFLUENZA VIRUSES AND USES THEREOF
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United States of America Patent
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Issued Date -
Nov 22, 2012
app pub date -
Nov 23, 2010
filing date -
Nov 23, 2009
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Abandoned
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Abstract
The present invention relates to nucleic acids that bind specifically to the matrix protein-1 of type A influenza viruses and uses thereof for detecting such viruses in a sample of interest. More particularly, the present invention relates to a nucleic acid that binds specifically to matrix protein-1 of type A influenza viruses characterized in that said nucleic acid comprises the following nucleotide sequence: 5′-N1-NS1-U-N3-A-NS3-NS5-NS7-NS6-CGCAU-NS4-C-N4-NS2-N2-3′ wherein: -N1 consists of a nucleotide -NS1 and NS2 consist of polynucleotides having 3 or 4 nucleotides in length, and NS1 and NS2 have complementary sequences; -N3 and N4 consists of a nucleotide, and N4 is complementary to N3; -NS3 and NS4 consist of polynucleotides having 3 nucleotides in length, and -NS3 and NS4 have complementary sequences -NS5 and NS6 consist of polynucleotides having 3 nucleotides in length, and -NS5 and NS6 have complementary sequences; -NS7 consists of a polynucleotide selected from the group consisting of AGAAUC (SEQ ID NO:12), UGAG (SEQ ID NO: 13), UAUUCC (SEQ ID NO:14), AGAU (SEQ ID NO:15), AGAATC (SEQ ID NO:16) or TGAG (SEQ ID NO:17) -N2 consists of a nucleotide that is complementary or not complementary to nucleotide N1.
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