Method for Specific Cleavage of N-CA Bond in Peptide Main Chain
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United States of America Patent
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Issued Date -
Nov 1, 2012
app pub date -
Jul 12, 2010
filing date -
Jul 16, 2009
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Abandoned
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Abstract
The present invention provides a peptide degradation reagent with the following characteristics: 1) it has no marked toxicity such as carcinogenicity, 2) it does not produce metastable peaks resulting from excessive degradation property, 3) it does not produce multiply-charged ion peaks which are interference peaks, and 4) it can secure separation and sharpness of peaks. The present invention also provides a method for specifically cleaving N—Cα bonds on a peptide backbone using the above-described reagent, and a method of determining the amino acid sequence of a peptide utilizing this specific cleavage. A method for specifically cleaving N—Cα bonds on the backbone of a peptide, comprising irradiating the peptide with laser light in the presence of 5-amino salicylic acid. A method for determining the amino acid sequence of a peptide, comprising irradiating the peptide with laser light in the presence of 5-amino salicylic acid to thereby specifically cleave N—Cα bonds on the peptide backbone. A reagent for specifically cleaving N—Cα bonds on a peptide backbone; a hydrogen radical-releasing reagent; a matrix reagent for MALDI-ISD; a matrix for MALDI-ISD; a peptide ionization reagent for MALDI-ISD; and a kit for MALDI-ISD.
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