Oxide Layers on Silicone Substrates for Effective Confocal Laser Microscopy
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United States of America Patent
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Issued Date -
Oct 18, 2012
app pub date -
Jun 26, 2012
filing date -
Dec 28, 2005
priority date (Note) -
Abandoned
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Abstract
Methods of performing confocal laser microscopy on a polymer array disposed on a silicon wafer substrate, the method comprising the steps of providing a silicon wafer substrate having a top side and a bottom side, coating the top side of the silicon wafer with an oxide coating to provide an oxide coated wafer, covalently coupling a plurality of probes to the top side of the coated wafer to provide a fixed polymer array, hybridizing the fixed polymer array with a plurality of labeled ligands, and assaying for one or more hybridized ligands using confocal laser fluorescence microscopy to detect hybridization are provided.
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