Methods for Determining Sequence Variants Using Ultra-Deep Sequencing

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United States of America Patent

SERIAL NO

13443629

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Abstract

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The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.

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Patent Owner(s)

Patent OwnerAddress
454 CORPORATION20 COMMERICAL STREET BRANFORD CT 06405

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Desany, Brian East Haven, US 2 274
Drake, James Branford, US 17 326
Egholm, Michael Madison, US 64 4043
Leamon, John Harris Guilford, US 3 603
Lee, William Lun Madison, US 2 274
Lohman, Kenton Guilford, US 2 274
Ronan, Michael Todd New Haven, US 3 287
Rothberg, Jonathan Guilford, US 79 3641
Simons, Jan Fredrik new Haven, US 10 1319

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