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Abstract

A method for automatic focusing of a microscope on a predetermined object in a specimen to be examined may include

a) producing a set of criteria to be satisfied for the predetermined object using at least one training image of the object,b) producing a first image of the specimen to be examined which contains the predetermined object with the microscope in a first focal position,c) ascertaining the section or sections of the first image, which in each case satisfies or satisfy the set of criteria according to step a), and defining each ascertained section as object area of the first image,d) producing further images of the specimen with the microscope in different focal positions,e) determining the optimum focal position(s) using the further images, wherein, for this purpose, in all images only the partial region or partial regions which correspond to the object area or object areas is/are evaluated,f) focusing the microscope on at least one of the optimum focal position(s) determined in step e).

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First Claim