REACTION MIXTURE FOR POLYMERASE CHAIN REACTION
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United States of America Patent
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Issued Date -
Sep 27, 2012
app pub date -
Dec 13, 2011
filing date -
Mar 23, 2011
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Abandoned
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Abstract
A reaction mixture for a polymerase chain reaction (PCR) includes water, DNA polymerase, deoxyribonucleoside triphosphates (dNTPs), tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), potassium chloride, magnesium chloride, dithiothreitol (DDT), and an additive agent. The reaction mixture is capable of enhancing the sensitivity and specificity of PCR and increasing the yield of correct nucleic acid sequence copies when being used in PCR.
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