FLOW CYTOMETRY METHOD THROUGH THE CONTROL OF FLUORESCENCE INTENSITIES
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United States of America Patent
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Issued Date -
Sep 13, 2012
app pub date -
Nov 24, 2010
filing date -
Nov 24, 2009
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Abstract
Provided is a flow cytometry method including adjusting cell populations targeted by antibodies conjugated with a same-color fluorochrome to show different fluorescence intensities according to types of the antibodies. Unlike a conventional flow cytometry method capable of classifying a positive and negative of one target using one antibody per color, the flow cytometry method adjusts several types of antibodies conjugated with a single-color fluorochrome to respectively show different fluorescence intensities, or adjusts the amounts of antibodies conjugated with a fluorochrome differently according to types of the antibodies, thereby classifying a positive and negative of multiple targets using one color. Accordingly, even when a current flow cytometer capable of classifying a limited number of colors is used, it is possible to classify a variety of cell populations to be clinically examined.
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