Method for Nutrient Pre-Loading of Microbial Cells
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United States of America Patent
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May 10, 2012
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Jun 24, 2009
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Abstract
A method is provided for supporting the growth of selected microbial cells and for obstructing the growth of contaminants in a non-sterile system. In the method, the microbial cells are pre-loaded with a surplus amount of a chosen nutrient, such as phosphorus, other macronutrients, or micronutrients. Further, the chosen nutrient is greatly reduced, or eliminated, from the non-sterile system. Thereafter, the pre-loaded selected microbial cells are introduced into the non-sterile system. In the non-sterile system, the selected microbial cells rely on the surplus amount of the chosen nutrient to survive and grow. At the same time, contaminants such as non-selected microbial strains and bacteria starve from a lack of the chosen nutrient in the non-sterile system.
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