NUCLEIC ACID AMPLIFICATION EMPLOYING TEMPERATURE OSCILLATION
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United States of America Patent
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Issued Date -
Aug 16, 2012
app pub date -
Sep 8, 2010
filing date -
Sep 8, 2009
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Abstract
A method for carrying out an isothermal nucleic acid amplification reaction at a predetermined temperature, said method comprising changing the temperature of the reaction mixture away from the said predetermined temperature and allowing it to return to the predetermined temperature at least once during the amplification reaction so as to cause a temperature oscillation in particular of up to 15° C., for example of from 2-10° C. Apparatus adapted to carry out the method forms a further aspect of the invention.
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