METHODS AND COMPOSITIONS FOR NUCLEIC ACID SAMPLE PREPARATION
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United States of America Patent
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Issued Date -
Aug 2, 2012
app pub date -
Jan 31, 2012
filing date -
Feb 2, 2011
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Abandoned
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Abstract
Provided are methods and compositions for the production of double-stranded nucleic acids, which can optionally be used as templates in high-throughput sequencing systems. In certain embodiments, these templates do not require exogenous primers to facilitate initiation of polymerase-dependent nascent strand synthesis. In certain embodiments, these templates comprise a single-stranded or gapped region that serves as a polymerase priming site.
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