Method for Producing Intracellular Microorganisms Using a Protozoan as a Host
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United States of America Patent
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Issued Date -
Jun 28, 2012
app pub date -
Jul 7, 2010
filing date -
Jul 7, 2009
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Abstract
The present invention relates to a method for producing intracellular microorganisms such as bacteria, parasitic protozoans or viruses, for example, characterised in that said method essentially comprises using a culture of at least one protozoan as a host cell in which said parasitic or non-parasitic intracellular microorganisms proliferate, and then recovering said intracellular microorganisms. The invention therefore proposes an alternative to selecting a mammal cell lineage for the in vitro culture of intracellular microorganisms. Said method preferably comprises at least the following steps: cultivating at least one protozoan strain in a suitable culture medium; then seeding the protozoan culture with at least one strain of an intracellular microorganism; followed by incubation, during which the intracellular microorganism develops in the cytoplasm of the protozoan until the latter lyses; and finally, recovering the produced intracellular microorganism.
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