A Lactobacillus plantarum 121-5 is disclosed. The preservation number is CGMCC No. 27418, and the Lactobacillus plantarum is preserved in the China general microbiological culture collection center on May 24, 2023. The Lactobacillus plantarum 121-5 has high beta-glucosidase activity, can efficiently convert polydatin to prepare resveratrol, and has short conversion time and high conversion rate. Meanwhile, the conversion condition is mild, safe and reliable, and the large-scale preparation is easy. The strain is directly applied to fermentation of foods and medicines (edible and medicinal plants or fruits and vegetables) containing polydatin, so that the resveratrol content in a fermentation product is improved, and the biological activity of the product is improved.

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