Bcl Gene Primers for Detection and Fingerprinting of Bacillus anthracis
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United States of America Patent
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Issued Date -
May 17, 2012
app pub date -
Nov 14, 2011
filing date -
Nov 15, 2010
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Abandoned
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Abstract
B. anthracis detection primers can be used to amplify the conserved regions of bclB alleles encoding the collagen-like proteins found in B. anthracis , as opposed to other Bacillus species, during PCR amplification of extracted Bacillus DNA or Bacillus spores. Additionally, B. anthracis strain fingerprinting primers amplify bclA-E polymorphic regions of collagen-like proteins found in B. anthracis strains. The B. anthracis strains differ in basepair size of these polymorphic coding regions of bclA-E so that strains can be discriminated based upon distinct PCR-band patterns that migrate differently in size while resolved on an agarose gel.
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