HOMOGENEOUS NONCOMPETITIVE DETECTION OF POST TRANSLATIONAL MODIFICATIONS FOR USE IN HIGH THROUGHPUT ASSAYS
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United States of America Patent
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Issued Date -
May 17, 2012
app pub date -
Nov 15, 2011
filing date -
Nov 16, 2010
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Abandoned
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Abstract
A non-competitive immunoassay method for detection of post-translationally modified (PTM) proteins is disclosed. The method will enable the direct detection of PTM proteins in solution or on solid phase with a single reagent addition step, and with improved selectivity for specific PTM sites on target proteins. The key to the method is the use of a secondary binding molecule that specifically recognizes the immune complex between the primary antibody and the antigen.
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