SELECTIVE ENZYMATIC AMIDATION OF C-TERMINAL ESTERS OR ACIDS OF PEPTIDES
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United States of America Patent
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Issued Date -
May 3, 2012
app pub date -
Jan 12, 2012
filing date -
Jun 25, 2007
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a process for the amidation of C-terminal esters or acids of peptide substrates in solution-phase synthesis of peptides, comprising amidating one or more peptide substrates comprising C-terminal esters or acids using the protease subtilisin in any suitable form in the presence of an ammonium salt derived from an acid having a pKa above 0.
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