HIGH-EFFICIENCY HOMOLOGOUS RECOMBINATION IN THE OIL-PRODUCING ALGA, NANNOCHLOROPSIS
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United States of America Patent
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Issued Date -
May 3, 2012
app pub date -
Sep 27, 2011
filing date -
Oct 19, 2009
priority date (Note) -
Abandoned
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Abstract
Transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting result in replacement of the target sequence of DNA with the sequence of DNA of interest.
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